Methods for analysis of Golgi complex function

Publisher Elsevier Science

Format

eBook

2nd Edition 1st Edition

Quantity

Add New

Publisher

Elsevier Science

Volume

Copyright

2013

ISBN13

9780124171831

Release

Format

eBook

Grade Level

College Freshman - College Senior

DDC
TBD

Overview

This new volume of Methods in Cell Biology looks at methods for analyzing of golgi complex function. Chapters\xa0cover such topics as in vitro reconstitution systems, fluorescence-based analysis of trafficking in mammalian cells and high content screening. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies. Chapters are written by experts in the field Cutting-edge material.
Front Cover.
Half Title Page.
Other Front Matter.
Title Page.
Copyright Page.
Contents.
Contributors.
Preface.
1: Model Systems and Functional Studies.
2: Analysis of GOLGI Complex Functions: In Vitro Reconstitution Systems.
3: RNA Interference Approaches to Examine GOLGI Function in Animal Cell Culture.
4: Trafficking along the Secretory Pathway in Drosophila Cell Line and Tissues: A Light and Electron Microscopy Approach.
5: Studying Membrane Trafficking in the Worm C elegans by RNA Interference.
6: Endomembrane and GOLGI Traffic in Plant Cells.
7: Biochemical Analysis of Secretory Trafficking in Mammalian Cells.
8: Methods for Analysis of Apical Lumen Trafficking Using Micropatterned 3d Systems.
9: Using Replication Defective Viruses to Analyze Membrane Trafficking in Polarized Epithelial Cells.
10: Snap-Tagging the Retrograde Route.
11: Assessing ER and GOLGI N-Glycosylation Process Using Metabolic Labeling in Mammalian Cultured Cells.
12: Imaging-Based Approaches.
13: Fluorescence-Based Analysis of Trafficking in Mammalian Cells.
14: Photobleaching Methods to Study GOLGI Complex Dynamics in Living Cells.
15: Photohighlighting Approaches to Access Membrane Dynamics of the GOLGI Apparatus.
16: Live Cell Visualization of GOLGI Membrane Dynamics by Super-Resolution Confocal Live Imaging Microscopy.
17: Analysis of GOLGI Complex Function Using Correlative Light-Electron Microscopy.
18: Viewing Golgi Structure and Function from a Different Perspective—insights from Electron Tomography.
19: High-Content Screening and Analysis of the GOLGI Complex.
20: Emerging Studies [New Frontiers].
21: (Re)Modeling the GOLGI.
22: Golgi Depletion from Living Cells with Laser Nanosurgery.
23: Studying Intracellular Trafficking Pathways with Probabilistic Density Maps.
24: Methods to Study Signaling at the GOLGI Apparatus.
25: Golgi-Dependent Signaling: Self-Coordination of Membrane Trafficking.
26: Signaling at the GOLGI during Mitosis.
27: Ice Recovery Assay for Detection of
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Methods for analysis of Golgi complex function

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Table of Contents